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From: Resilient Planet Mission 1: Invaders (pp: 22)
Cycling Carbon

In this lab, students will investigate the release of carbon-containing gas from living and nonliving sources.

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Cave

Russell Cuhel collects and analyzes water samples to discover how abiotic factors affect the ecology of Lake Michigan. One of the elements he monitors is carbon, which cycles between living and nonliving parts of the biosphere. In organisms, carbon is a major component in the chemical structure of organic compounds. We also find carbon-based molecules in rocks, dissolved in water, and as gases in the atmosphere. When carbon dioxide mixes with water, it produces an acid, which can be detected by a chemical indicator.

In this activity, you will investigate the release of carbon-containing gas from living and nonliving sources.


Materials
  • Lab 4 Data Sheet
  • straw
  • one 250 ml beaker
  • Bromothymol Blue
  • safety goggles
  • lab apron
  • gloves
  • nail
  • paper towel
  • vinegar
  • lima bean seeds
  • hand lens
  • limestone rock
  • plastic tape
  • one medium-size balloon

Caution

Review all safety precautions with your instructor before beginning this lab. When working with chemicals, always wear goggles, gloves, and aprons.


Lab Prep
  1. Review the carbon cycle diagram on page 21. Describe the interactions you find in the cycling of carbon. Compare and contrast the role of green plants with that of animals in carbon cycling.

Make Observations
  1. Put on your safety goggles, apron, and gloves.
  2. Limestone is a natural sink of carbon. Carbon is only released from limestone when the rock undergoes a chemical change. Obtain a piece of limestone rock and place it on the paper towel. Use a nail to make small scratches on the surface of the rock.
  3. Place a few drops of vinegar on the scratched area. Using a hand lens, observe the bubbles caused by the carbon dioxide gas that is released by this chemical reaction.
  4. Insert straw into the neck of the balloon. Tape it tight to create an air-tight seal.
  5. Put 50 mL of tap water into the beaker. Add 20 drops of Bromothymol Blue. Gently swirl and note the color of the solution.
  6. Blow into the straw to fill the balloon and pinch the straw near the neck of the balloon to keep the air from escaping. Place the open end of the straw into the water. Slowly release the straw to allow the air in the balloon to bubble into the solution. Record the changes. Explain what caused the changes.
  7. Conduct a thought experiment - what would happen if you added an aquatic plant to that beaker?
  8. Based on the methods you just learned for detecting carbon, design an experiment that would demonstrate a plant giving off carbon dioxide.
Journal Question

Journal Question
How could you test carbonated beverages to show that the dissolved gas was carbon dioxide?

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